货号：E-2059967
CAS：N/A
规格：mg
纯度：≥95%
生产商：瑞禧生物

FAM azide| FAM N3|FAM叠氮化物
FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.
With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.
FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.
   
FAM azide| FAM N3|FAM叠氮化物
 
 
General properties：
Appearance:

yellowish crystals
Molecular weight:

458.42
Molecular formula:

C24H18N4O6
Solubility:

soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control:

NMR 1H, HPLC-MS (95%)
Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
 
Spectral properties：
Excitation maximum, nm:

494
ε, L⋅mol−1⋅cm−1:

75000
Emission maximum, nm:

520
Fluorescence quantum yield:

0.9
CF260:

0.20
CF280:

0.17
 
荧光染料示例

激发光
Indo-1, Ca saturated

331 nm
Indo-1 Ca2+

346 nm
Cascade Blue BSA pH 7.0

401 nm
Cascade Blue

398 nm
LysoTracker Blue

373 nm
Alexa 405

401 nm
LysoSensor Blue pH 5.0

374 nm
LysoSensor Blue

374 nm
DyLight 405

399 nm
DyLight 350

332 nm
BFP (Blue Fluorescent Protein)

380 nm
Alexa 350

343 nm
7-Amino-4-methylcoumarin pH 7.0

346 nm
Amino Coumarin

345 nm
AMCA conjugate

347 nm
Coumarin

360 nm
7-Hydroxy-4-methylcoumarin

360 nm
7-Hydroxy-4-methylcoumarin pH 9.0

361 nm
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0

358 nm
Hoechst 33342

352 nm
Pacific Blue

404 nm
Hoechst 33258

352 nm
Hoechst 33258-DNA

352 nm
Pacific Blue antibody conjugate pH 8.0

404 nm
PO-PRO-1

434 nm
PO-PRO-1-DNA

435 nm
POPO-1

433 nm
POPO-1-DNA

433 nm
DAPI-DNA

359 nm
DAPI

358 nm
Marina Blue

362 nm
SYTOX Blue-DNA

445 nm
CFP (Cyan Fluorescent Protein)

434 nm
eCFP (Enhanced Cyan Fluorescent Protein)

437 nm
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS)

375 nm
Indo-1, Ca free

346 nm
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid)

375 nm
BO-PRO-1-DNA

462 nm
BOPRO-1

462 nm
BOBO-1-DNA

461 nm
SYTO 45-DNA

451 nm
evoglow-Pp1

448 nm
evoglow-Bs1

448 nm
evoglow-Bs2

448 nm
Auramine O

431 nm
DiO

487 nm
LysoSensor Green pH 5.0

447 nm
Cy 2

489 nm
LysoSensor Green

447 nm
Fura-2, high Ca

336 nm
Fura-2 Ca2+sup>

336 nm
SYTO 13-DNA

488 nm
YO-PRO-1-DNA

491 nm
YOYO-1-DNA

491 nm
eGFP (Enhanced Green Fluorescent Protein)

488 nm
LysoTracker Green

503 nm
GFP (S65T)

489 nm
BODIPY FL, MeOH

502 nm
Sapphire

396 nm
BODIPY FL conjugate

503 nm
MitoTracker Green

490 nm
MitoTracker Green FM, MeOH

490 nm
Fluorescein 0.1 M NaOH

493 nm
Calcein pH 9.0

494 nm
Fluorescein pH 9.0

490 nm
Calcein

493 nm
Fura-2, no Ca

367 nm
Fluo-4

494 nm
FDA

495 nm
DTAF

495 nm
Fluorescein

495 nm
Fluorescein antibody conjugate pH 8.0

493 nm
CFDA

495 nm
FITC

495 nm
Alexa Fluor 488 hydrazide-water

493 nm
DyLight 488

493 nm
5-FAM pH 9.0

492 nm
FITC antibody conjugate pH 8.0

495 nm
Alexa 488

493 nm
Rhodamine 110

497 nm
Rhodamine 110 pH 7.0

497 nm
Acridine Orange

431 nm
Alexa Fluor 488 antibody conjugate pH 8.0

499 nm
BCECF pH 5.5

485 nm
PicoGreendsDNA quantitation reagent

502 nm
SYBR Green I

498 nm
Rhodaminen Green pH 7.0

497 nm
CyQUANT GR-DNA

502 nm
NeuroTrace 500/525, green fluorescent Nissl stain-RNA

497 nm
DansylCadaverine

335 nm
Rhodol Green antibody conjugate pH 8.0

499 nm
Fluoro-Emerald

495 nm
Nissl

497 nm
Fluorescein dextran pH 8.0

501 nm
Rhodamine Green

497 nm
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0

504 nm
DansylCadaverine, MeOH

335 nm
eYFP (Enhanced Yellow Fluorescent Protein)

514 nm
Oregon Green 488

498 nm
Oregon Green 488 antibody conjugate pH 8.0

498 nm
Fluo-3

506 nm
BCECF pH 9.0

501 nm
SBFI-Na+

336 nm
Fluo-3 Ca2+

506 nm
Rhodamine 123, MeOH

507 nm
FlAsH

509 nm
Calcium Green-1 Ca2+

506 nm
Magnesium Green

507 nm
DM-NERF pH 4.0

493 nm
Calcium Green

506 nm
Citrine

515 nm
LysoSensor Yellow pH 9.0

335 nm
TO-PRO-1-DNA

515 nm
Magnesium Green Mg2+

507 nm
Sodium Green Na+

507 nm
TOTO-1-DNA

514 nm
Oregon Green 514

512 nm
Oregon Green 514 antibody conjugate pH 8.0

513 nm
NBD-X

466 nm
DM-NERF pH 7.0

509 nm
NBD-X, MeOH

467 nm
CI-NERF pH 6.0

513 nm
Alexa 430

431 nm
Alexa Fluor 430 antibody conjugate pH 7.2

431 nm
CI-NERF pH 2.5

504 nm
Lucifer Yellow, CH

428 nm
LysoSensor Yellow pH 3.0

389 nm
6-TET, SE pH 9.0

521 nm
Eosin antibody conjugate pH 8.0

525 nm
Eosin

524 nm
6-Carboxyrhodamine 6G pH 7.0

526 nm
6-Carboxyrhodamine 6G, hydrochloride

525 nm
Bodipy R6G SE

528 nm
BODIPY R6G, MeOH

528 nm
6 JOE

520 nm
Cascade Yellow antibody conjugate pH 8.0

399 nm
Cascade Yellow

399 nm
mBanana

540 nm
Alexa Fluor 532 antibody conjugate pH 7.2

528 nm
Alexa 532

528 nm
Erythrosin-5-isothiocyanate pH 9.0

533 nm
6-HEX, SE pH 9.0

534 nm
mOrange

548 nm
mHoneydew

478 nm
Cy 3

549 nm
Rhodamine B

543 nm
DiI

551 nm
5-TAMRA-MeOH

543 nm
Alexa 555

553 nm
Alexa Fluor 555 antibody conjugate pH 7.2

553 nm
DyLight 549

555 nm
BODIPY TMR-X, SE

544 nm
BODIPY TMR-X, MeOH

544 nm
PO-PRO-3-DNA

539 nm
PO-PRO-3

539 nm
Rhodamine

551 nm
Bodipy TMR-X conjugate

544 nm
POPO-3

533 nm
Alexa 546

562 nm
BODIPY TMR-X antibody conjugate pH 7.2

544 nm
Calcium Orange Ca2+

549 nm
TRITC

550 nm
Calcium Orange

549 nm
Rhodaminephalloidin pH 7.0

558 nm
MitoTracker Orange

551 nm
MitoTracker Orange, MeOH

551 nm
Phycoerythrin

565 nm
Magnesium Orange

550 nm
R-Phycoerythrin pH 7.5

565 nm
5-TAMRA pH 7.0

553 nm
5-TAMRA

549 nm
Rhod-2

552 nm
FM 1-43

472 nm
Rhod-2 Ca2+

553 nm
Tetramethylrhodamine antibody conjugate pH 8.0

552 nm
FM 1-43 lipid

473 nm
LOLO-1-DNA

568 nm
dTomato

554 nm
DsRed

563 nm
Dapoxyl (2-aminoethyl) sulfonamide

372 nm
Tetramethylrhodamine dextran pH 7.0

555 nm
Fluor-Ruby

554 nm
Resorufin

571 nm
Resorufin pH 9.0

571 nm
mTangerine

568 nm
LysoTracker Red

578 nm
Lissaminerhodamine

572 nm
Cy 3.5

578 nm
Rhodamine Red-X antibody conjugate pH 8.0

573 nm
Sulforhodamine 101, EtOH

578 nm
JC-1 pH 8.2

593 nm
JC-1

592 nm
mStrawberry

575 nm
MitoTracker Red

578 nm
MitoTracker Red, MeOH

578 nm
X-Rhod-1 Ca2+

580 nm
Alexa Fluor 568 antibody conjugate pH 7.2

579 nm
Alexa 568

576 nm
5-ROX pH 7.0

578 nm
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt)

578 nm
BO-PRO-3-DNA

574 nm
BOPRO-3

574 nm
BOBO-3-DNA

570 nm
Ethidium Bromide

524 nm
ReAsH

597 nm
Calcium Crimson

589 nm
Calcium Crimson Ca2+

590 nm
mRFP

585 nm
mCherry

587 nm
Texas Red-X antibody conjugate pH 7.2

596 nm
HcRed

590 nm
DyLight 594

592 nm
Ethidium homodimer-1-DNA

528 nm
Ethidiumhomodimer

528 nm
Propidium Iodide

538 nm
SYPRO Ruby

467 nm
Propidium Iodide-DNA

538 nm
Alexa 594

590 nm
BODIPY TR-X, SE

588 nm
BODIPY TR-X, MeOH

588 nm
BODIPY TR-X phallacidin pH 7.0

590 nm
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2

567 nm
YO-PRO-3-DNA

613 nm
Di-8 ANEPPS

469 nm
Di-8-ANEPPS-lipid

469 nm
YOYO-3-DNA

612 nm
Nile Red-lipid

553 nm
Nile Red

559 nm
DyLight 633

624 nm
mPlum

587 nm
TO-PRO-3-DNA

642 nm
DDAO pH 9.0

648 nm
Fura Red, high Ca

434 nm
Allophycocyanin pH 7.5

651 nm
APC (allophycocyanin)

650 nm
Nile Blue, EtOH

631 nm
TOTO-3-DNA

642 nm
Cy 5

646 nm
BODIPY 650/665-X, MeOH

646 nm
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2

569 nm
DyLight 649

652 nm
Alexa Fluor 647 antibody conjugate pH 7.2

653 nm
Alexa 647

653 nm
Fura Red Ca2+

435 nm
Atto 647

644 nm
Fura Red, low Ca

472 nm
Carboxynaphthofluorescein pH 10.0

600 nm
Alexa 660

664 nm
Alexa Fluor 660 antibody conjugate pH 7.2

663 nm
Cy 5.5

673 nm
Alexa Fluor 680 antibody conjugate pH 7.2

679 nm
Alexa 680

679 nm
DyLight 680

678 nm
Alexa Fluor 700 antibody conjugate pH 7.2

696 nm
Alexa 700

696 nm
FM 4-64, 2% CHAPS

506 nm
FM 4-64

508 nm